RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues.
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RNase A (Ribonuclease A) is a pancreatic endoribunuclease (13.7kDa monomer) that specifically cleaves single-stranded RNA at the 3′ end of pyrimidine residues. The phosphodiester bond between the 5′-ribose of one nucleotide and the phosphate group of the 3′-ribose of an adjacent pyrimidine nucleotide is broken and the resulting 2′, 3′-cyclic phosphate is hydrolyzed to the corresponding 3′-nucleoside phosphate.
Applications
Plasmid DNA preparations, genomic DNA preparations, Ribonuclease protection assays, RNA removal from recombinant protein preparations, single-base mutation mapping in DNA or RNA by mismatch cleavage