proACE™ Green DNA Stain

proACE™ Green DNA Stain is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for in-gel staining and post-staining and is compatible with both UV and Blue LED transilluminators.

Properties:
proACE™ Green DNA Stain has fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and in the blue/green light range (470-488nm and 510-514nm). Maximum fluorescence emission is at 527-533nm (green). Therefore, proACE™ Green DNA Stain is compatible with a large variety of gel documentation systems. Yellow/orange filters should be used for photography.

Sensitivity:
The detection limit of proACE™   Green DNA Stain is in the range of 0.5-2.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc). proACE™ Green DNA Stain is also suitable for post-staining.                          

Safety:
proACE™ Green DNA Stain is non-mutagenic as determined by the Ames-test. Moreover, genotoxicity analysis shows negative results for both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermatocyte chromosomal aberration test. The complete safety report can be found on the product page at our website. proACE™ Green DNA Stain is non-hazardous; however, one should always exercise common safe laboratory practices. Use goggles and gloves as proACE™ Green DNA Stain may cause skin or eye irritations.

Agarose Gels (pre-staining)

1. Prepare agarose solution by mixing the desired amount of agarose and desired volume of electrophoresis buffer, and dissolving the agarose by heating.
2. Once the solution has become clear, remove from the microwave or heater, swirl gently, and allow the solution to cool down to ~60°
3. For each 100ml of agarose solution, add 5µl of proACE™ Green DNA Stain

(for example:  2µl for a 40ml gel)

4. Mix gently by swirling. The solution should be devoid of air bubbles. Pour to cast the gel.
5. Run gel according to normal procedure.
6. Visualize using either UV or Blue LED light.

Agarose Gels (post-staining)

1. Prepare agarose solution by mixing the desired amount of agarose and desired volume of electrophoresis buffer, and dissolving the agarose by heating.
2. Once the solution has become clear, remove from the microwave or heater, swirl gently and allow the solution to cool down to ~60° Pour to cast the gel.
3. Run gel according to normal procedure.
4. Submerge the gel in post-staining solution that contains 3µl of proACE™ Green DNA Stain per 10ml of Electrophoresis Buffer. Ensure you use sufficient buffer to cover the gel completely.
5. Agitate gently, protected from light, at room temperature, for 30 minutes.
6. Visualize using either UV or Blue LED light.
7. The leftover staining solution can be stored, for re-use, in a sealed container, protected from light, at room temperature, for up to 1 week.