High-fidelity 2× Mastermix with ~100× lower error rate than Taq. Reversible aptamer hotstart, enhanced processivity. Ideal for NGS library prep, cloning, site-directed mutagenesis, and long-range PCR.
Xpert ampliFi Hotstart 2X Mastermix is based on a Pfu-derived enzyme with an error rate ~100× lower than standard Taq. Features enhanced DNA binding for improved processivity, shorter extension times, and superior performance on complex templates including GC/AT-rich sequences. A reversible aptamer-based hotstart prevents non-specific amplification. Ideal for site-directed mutagenesis, NGS library prep, cloning, and long-range PCR.