Discovering Answers • Unraveling Nature
Single-tube enzymatic PCR clean-up combining Exonuclease I and recombinant SAP. Removes primers and dNTPs in 5 minutes; sequencing-ready in 15 minutes with 100% recovery.
Xpert Exo/SAP 1tube is an enzymatic PCR clean-up kit comprising Exonuclease I (Exo I) and recombinant Shrimp Alkaline Phosphatase (rSAP) in an optimal molar ratio. Exo I degrades residual primers whilst rSAP dephosphorylates excess dNTPs — both in a single 5-minute reaction. Both enzymes are subsequently heat-inactivated, and the DNA is sequencing-ready in just 15 minutes with 100% recovery, even for very short PCR products. No purification columns or precipitation steps required.
